Doug DeHaven As a photographer working with microscopy, I often encounter the challenge of limited depth of field when capturing images of intricate specimens. Toph address this, I employ a technique called focus stacking. By capturing a series of images of the same specimen at various focal planes, each emphasizing different parts of the subject, I can extend the depth of field far beyond what is achievable with a single image. Using Adobe Photoshop and specialized software, I align and merge these images, selectively blending areas of sharp focus to create a final composite image with a significantly enhanced depth of field. This process allows me to reveal fine details and subtle structures within my samples that would otherwise be lost due to the limitations of optical systems. Focus stacking thus serves as an invaluable tool in my research, enabling me to produce clear and comprehensive images essential for studying the intricacies of unique specimens at high magnification.
Here is a video passing through the entire depth of the rust formation, capturing an image at each step as I go.
And the resulting image.
